Development of a molecular marker for the identification of lampreys at the juvenile stage.
References
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Abstract
There are three species of lamprey in France: the sea lamprey (Petromyzon marinus), the river lamprey (Lampetra fluviatilis) and the brook lamprey (L.planeri). The sea lamprey is classified as endangered and the river lamprey as vulnerable, according to criteria of the International Union for Conservation of Nature (IUCN), on the French red list of fishes. The distribution of the three species remains poorly known in France, and this project initially aimed to develop an approach for detecting the three species using environmental DNA (eDNA). A first step was the development of molecular markers to distinguish these species. In particular, as the brook lamprey and the river lamprey are morphologically indistinguishable at the juvenile stage, we developed a molecular tool for discriminating between these two species. As a result, it is now possible to identify both species from a fin clip sampled non-lethally on a larva (known as 'ammocoete') collected in the field. This approach will allow a better understanding of the distribution of lamprey species, and in particular the growth zones of ammocoetes that spend several years buried in the soft substrate of river beds.
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